polycloning site

polycloning site
(= multiple cloning site; MCS)
Region of a phage or plasmid vector that has been engineered to contain a series of restriction sites that are usually unique within the entire vector. This makes it particularly easy to insert or excise (subclone) DNA fragments.

Dictionary of molecular biology. 2004.

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Look at other dictionaries:

  • polycloning site — See: polylinker …   Glossary of Biotechnology

  • multiple cloning site — See polycloning site …   Dictionary of molecular biology

  • blue-white colour selection — Method for identifying bacterial clones containing plasmids with inserts. Many modern vectors have their polycloning site within a part of the LacZ gene encoding beta galactosidase, which provides a complementation in an appropriate mutant E.… …   Dictionary of molecular biology

  • polylinker — An inserted sequence of DNA in recombinant DNA vectors consisting of a cluster of numerous restriction endonuclease sites unique in the plasmid; also called restriction site bank and polycloning site …   Medical dictionary

  • MCS — See polycloning site …   Dictionary of molecular biology

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